Validation of a maximal incremental skating test performed on a slide board: comparison with treadmill skating Post-print as accepted for publication. Original Investigation *Tatiane Piucco1, 2, Fernando Diefenthaeler1, 2, Rogério Soares1, Juan M. Murias1, Guillaume Y. Millet1 1 Faculty of Kinesiology, University of Calgary, Calgary, Canada. 2500 University Drive NW, Calgary, AB, Canada T2N 1N4 2 Sports Center, Federal University of Santa Catarina, Florianópolis, Brazil. Campus Universitário, Trindade, Florianópolis, SC, Brazil CEP: 88040-900 *Corresponding author: University of Calgary, Calgary, Canada Faculty of Kinesiology – Human Performance Laboratory 2500 University Drive NW, Calgary, AB, Canada T2N 1N4 Phone: +1 (403) 220-6472 | Fax: 403-284-3553 Email: tatianepiucco@yahoo.com.br Running head: Maximal skating test on slide board Abstract word count 266 Word count 3491 Number of Tables 2 Number of Figures 4 1 Validation of a maximal incremental skating test performed on a slide board: comparison with treadmill skating Abstract Purpose: the aim of this study was to investigate the criterion validity of a maximal incremental skating test performed on a slide board (SB). Methods: Twelve sub-elite speed skaters performed a maximal skating test on a treadmill and on a SB. Gas exchange threshold (GET), respiratory compensation point (RCP) and maximal variables were determined. Results: oxygen uptake (V̇O2) (31.0 ± 3.2 and 31.4 ± 4.1 mL∙min-1∙kg-1), percentage of maximal oxygen uptake (V̇O2max) (66.3 ± 4 and 67.7 ± 7.1%), HR (153 ± 14 and 150 ± 12 bpm), and ventilation (59.8 ± 11.8 and 57.0 ± 10.7 L∙min-1) at GET, and V̇O2 (42.5 ± 4.4 and 42.9 ± 4.8 mL∙min-1∙kg-1), percentage of V̇O2max (91.1 ± 3.3 and 92.4 ± 2.1%), heart rate (HR) (178 ± 9 and 178 ± 6 bpm), and ventilation (96.5 ± 19.2 and 92.1 ± 12.7 L∙min-1) at RCP were not different between skating on a treadmill and on a SB. V̇O2max (46.7 ± 4.4 vs 46.4 ± 6.1 mL∙min-1∙kg-1) and maximal HR (195 ± 6 vs 196 ± 10 bpm) were not significantly different and correlated (r = 0.80 and r = 0.87, respectively; p < 0.05) between the treadmill and SB. V̇O2 at GET, RCP and V̇O2max obtained on a SB were correlated (r > 0.8) with athletes’ best time on 1500 m. Conclusions: the incremental skating test on a SB was capable to distinguish maximal (V̇O2 and HR) and submaximal (V̇O2, % V̇O2max, HR and ventilation) parameters known to determine endurance performance. Therefore, the SB test can be considered as a specific and practical alternative to evaluate speed skaters. Keywords: speed skating; incremental test; test validity; intensity thresholds; maximal oxygen uptake 2 Introduction A maximal incremental exercise test is a well-established method for determining key parameters of aerobic capacity in humans, such as gas exchange threshold (GET), respiratory compensation point (RCP), and maximal oxygen uptake (V̇O2max). Since indices of aerobic capacity such as V̇O2max and physiological exercise intensity thresholds are associated with performance during speed skating competitions1, a skating-specific incremental test is needed to properly measure these parameters. Speed skating is characterized by a unique crouched position and side-ways movement of the body that determine the physiological demands. The low posture adopted during speed skating and the long isometric phase of each stroke, followed by concentric phase, results in restriction of blood flow to the lower limbs and high intramuscular forces2,3. These specific characteristics are associated with a lower V̇O2max and higher maximal heart rate (HRmax) during skating when compared with running or cycling4,5,6. Despite the lack of specificity, cycle ergometer or treadmill running tests are widely used in speed skating7 for monitoring physiological changes and establishing training intensities. Optimal training adaptations can be obtained from training loads specifically related to the activity itself, due to the specific physiological and neuromuscular demands8. As a result, performance evaluations for exercise prescription must be movement specific, valid and reliable 9. Nobes et al.10 found no difference in V̇O2max achieved during a maximal skating protocol on treadmill and on ice, suggesting that skating treadmill could be of great value to evaluate speed skaters. However, skating treadmills are very expensive and the athletes need to be highly-skilled and familiar with skating on a treadmill to be able to perform maximally. All these limitations challenge the use of skating treadmills for optimizing training programs through periodic laboratory evaluation. Skating protocols performed on ice track are even more complicated to perform, as testing conditions are more difficult to control. The necessity of having long periods of test interruptions to collect blood samples may increase data variability which would affect the reliability of the results, limiting the application of incremental cardiorespiratory tests on track4,5 Moreover, at maximal intensity, it may become biomechanically or technically difficult to skate fast enough to fully challenge the cardiovascular system11. As an alternative, some researchers investigated physiological responses obtained during low walking on an oversized motor-driven treadmill, which simulated the posture used in speed skating12. Another study investigated physiological responses during a maximal skating test on a slide board13. However, the aforementioned studies did not determine the validity of those protocols compared with a real skating activity. Given the importance of assessing different parameters of aerobic function to monitor speed skaters, and considering the technical and costrelated limitations of other testing modalities, the use of a slide board based incremental test to determine endurance performance in a more accessible, valid and laboratory controlled way is warranted. Therefore, the purpose of this study was to assess the validity of submaximal and maximal aerobic indices related to endurance performance during an incremental test performed on slide board, compared to a similar skating protocol on treadmill. The main hypothesis was that skating on the slide board would elicit similar submaximal and maximal cardiorespiratory responses compared to skating on the treadmill. Methods 3 Participants Twelve (4 male and 8 female) long-track speed skaters (distances between 500 and 5000 m) voluntarily participated in this study. The athlete’s mean age, body mass, and height were 18.0 ± 0.9 years, 65.0 ± 6.8 kg, and 1.73 ± 8.80 m, respectively. The speed skaters participated in a systematic training program with a volume of 2 hours/day, 5 days per week, for at least 3 years. According to indicators based on current and past training data14, this group of individuals fit within the “trained” category and performance level 4. The tests were performed during the competitive season. The skaters’ best time average for the 1500 m distance was 2.13 ± 0.14 min. The study was conducted in accordance with ethical standards of the local University Human Research Ethics Committee (REB15-2537), and all participants signed an informed consent form with detailed description about the study protocol. Design Participants were instructed to refrain from heavy exercise (any physical activity that was beyond their activities of daily living or a recovery-like exercise session) in the 24 h before each test, maintain a similar diet, and to abstain from the ingestion of stimulants (i.e., caffeine, nicotine) or alcohol. Each subject performed two incremental skating tests to fatigue, separated by two to four days. One test was performed on a treadmill and the other on an instrumented slide board. All participants were familiarized with the treadmill skating protocol, using their own inline skates, at least twice, for a minimum of 30 min 2 to 5 days before the data collection. Participants were familiar with the slide board skating movement as they use it for training. Methodology A maximal inline skating protocol was performed on an oversized, motor driven treadmill (2.5 × 3.5 m) (Athletic Republic, Salt Lake City, UT). During the test the athletes wore a safety harness that was attached to an overhead pulley as a precaution to prevent from a potential fall. After 3 min of baseline skating at 8 km·h-1, the treadmill skating protocol started at 12 km·h-1, and increased by 2 km·h-1 every minute until volitional exhaustion despite strong verbal encouragement. We opted to not to change the treadmill grade in order to provide a condition as close as possible to real skating and slide board skating exercises. Because our subjects were well-trained skaters familiar with treadmill skating, they did not have any technical difficulties to skate at high velocities. Therefore, maximum effort was attained and the athletes stopped due to exhaustion, not technical limitations. A slide board equipped with sensors and connected to custom made software was used for a maximal incremental test, as described elsewhere13. Briefly, each athlete skated on a slide board of polyethylene surface (2.0 × 0.6 × 0.2 m) wearing a pair of nylon socks over their shoes while skating. Optical sensors were placed at either extremity of the slide board to determine the athletes’ instantaneous skating cadence and a software program was developed to help the athlete keep the pace by providing visual and auditory feedback (Figure 1). After 3 min of baseline skating at 15 push-offs per minute (ppm), the protocol started with a cadence of 30 ppm, and increased by 3 ppm every minute until volitional exhaustion occurred despite strong verbal encouragement (Figure 2). The reliability of this protocol has been previously determined (ICC > 0.9 and typical error of measure expressed as a coefficient of variation < 3.5%)15. Throughout each exercise trial pulmonary ventilation (V̇E), respiratory exchange ratio (RER), carbon dioxide production (V̇CO2) and oxygen consumption (V̇O2) were measured breath-bybreath using a portable gas analyzer (K4b2 Cosmed®, Rome, Italy), calibrated according to 4 manufacturer’s instructions prior to each test. V̇O2max was considered to be the highest averaged value over a 15-second period during the last stage of the test. HR was collected using radiotelemetry (SP0180 Polar Transmitter; Polar Electro Inc., Kempele, Finland). Blood samples from the fingertip were collected at the end of each test, and at minute one, three and five following the conclusion of the test, to assess peak of blood lactate concentration ([La]peak). The Lactate Scout (SensLab GmbH, Leipzig, Germany) analyser was used and calibrated according to the manufacturer’s recommendations. The gas exchange threshold (GET) and the respiratory compensation point (RCP) were identified by two blinded experts. If the two reviewers agree within discrepancy of no more than 200 mL∙min-1 then the average will result in an error no larger than 100 mL∙min-1, which would be within the lowest detectable noise in the V̇O2 data16. Only when a discrepancy was larger than 200 mL∙min-1 and the potential error was unacceptable, a third reviewer was involved to discuss the thresholds. GET was determined by visual inspection as the V̇O2 at which V̇CO2 began to increase out of proportion in relation to V̇O2, with a systematic rise in V̇E-to-V̇O2 relation and end-tidal partial pressure of O2 (PO2) whereas the ventilatory equivalent of V̇CO2 (V̇E/V̇CO2) and end-tidal partial pressure of CO2 (PCO2) is stable17. RCP was determined as the point where end-tidal PCO2 began to fall after a period of isocapnic buffering18. This point was confirmed by examining V̇E/V̇CO2 plotted against V̇O2 and by identifying the second breakpoint in the V̇E-to-V̇O2 relation. Maximal cadence at the slide board (CADmax) was defined as the maximal number of push-offs per minute reached during the slide board test. If the final stage was not completed, the CADmax was calculated according to the following equation adapted from Kuipers et al.19: CADmax = CADf + t/s × 3, where CADf is the cadence of the final stage completed, t the uncompleted stage time (in s), s the stage duration (= 60 s) and 3 the cadence increment per stage. The same equation was used to calculate maximal speed at the end of the treadmill skating test, using speed values instead of cadence and 2 (increments of 2 km·h-1) instead of 3. Statistical Analysis Data are presented as means ± SD. Normality of the data was assessed using the ShapiroWilk test. Two-tailed pairwise t-tests were performed to compare variables obtained from a maximal incremental test performed on a skating treadmill with variables obtained from a maximal incremental test performed on a slide board. Effect sizes (ES) of each pair of comparisons were categorized as small (ES < 0.20), moderate (ES > 0.20 - 0.8) or large effects (ES > 0.8)20. Pearson correlation coefficients between the variables obtained from the treadmill and slide board protocols and between the skaters’ best time on 1500 m distance on ice and GET, RCP and V̇O2max. The following criteria were adopted for interpreting the magnitude of correlation between variables: <0.10, trivial; 0.11–0.30, small; 0.31–0.50, moderate; 0.51–0.70, large; 0.71–0.90, very large; and 0.91–1.00, almost perfect21. A level of magnitude above very large is warranted for correlations, because a value of 0.9 is described as a threshold for validity21. Typical error of measurement, expressed as absolute values and as coefficient of variation (TEMCV%), were determined using the techniques described by Hopkins21. Statistical analysis was conducted using GraphPad Prism (GraphPad Prism Software Inc. v. 5.0, San Diego, CA) and Statistical Package for Social Sciences (SPSS Inc. v.17.0, Chicago, IL). The statistical significance was accepted when p<0.05. Results Maximal physiological comparisons indices and correlation are reported in table 1. No statistically significant differences were found for V̇O2max and HRmax measured when skating on a treadmill versus skating on a slide board. [La]peak, RERmax and VEmax were significantly higher 5 when skating on a treadmill. Significant correlations were obtained for V̇O2max (r = 0.94), HRmax (r = 0.87) and V̇Emax (r = 0.87) (Table 1). Maximal cadence (60 ± 4.8 ppm) and maximal speed (30.7 ± 4.1 km·h-1) values were significantly correlated (r = 0.77). Table 2 depicts physiological responses associated to the GET and RCP during the maximal incremental skating tests on a treadmill and on a slide board. The V̇O2, percentage of V̇O2max (%V̇O2max), HR, and V̇E values associated with GET and RCP were not significant different between the skating on a treadmill and on a slide board. RER values at GET and RCP were significantly smaller on a slide board skating. Most of the variables at GET and RCP obtained on a treadmill and on a slide board skating were significantly correlated, except %V̇O2max at RCP and RER at GET and RCP intensities. Very large correlation was found for V̇O2max attained during treadmill and slide board skating (Figure 3), and very similar V̇O2 responses were obtained throughout the two exercise modalities (Figure 4). Very large correlations were found between the athletes’ personal best time on 1500 m distance (2.13 ± 0.14 min) and V̇O2max in L∙min-1 (r = 0.85; 0.91), GET (r = 0.81; 0,80) and RCP (r = 0.88; 0,89) found during skating on a treadmill and on a slide board, respectively. Discussion This study aimed to compare physiological responses during a maximal incremental skating test on a treadmill and on a slide board. The main finding was that the V̇O2max and V̇O2 associated with GET and RCP were not statistically different and significantly correlated between both exercise modalities, suggesting that skating on a slide board is a good representation of the physiological responses obtained during treadmill skating. To deliver high power outputs, both aerobic and anaerobic resources are crucial, although their contribution varies over the different distances22. The averaged V̇O2max values found on a slide board were similar and correlated to those obtained on a skating treadmill (Table 1). These results are similar to the V̇O2max found during two different slide board skating protocols performed by speed skaters of similar age and training volume13. It is known that V̇O2max values reported during skating are around 7-10% lower than those during cycling, likely due to the physiological consequences of the skating position2,3,7. Consequently, the relatively low V̇O2max values reported in the present study are not surprising, considering the low V̇O2max values previously reported in the literature for elite speed skaters tested on ice (53.9 mL∙kg-1∙min-1 for males) and on a cycle ergometer (57.2–62.0 and 52.2–54.9 mL∙kg-1∙min-1 for males and females, respectively)1,6,8. Moreover, the skaters evaluated in the present study were not elite athletes, mainly females (8/12) and most of them short distance specialists (under 3000 m). The average V̇O2 responses during the two protocols were virtually the same (Figure 4A and B), indicating a close pattern of physiological demand. A relatively large plateau in V̇O2 versus workload relationship was observed for most of the tests. Foster et al.23 pointed out to a remarkable ability of the skaters to rapidly attain and sustain maximal level of V̇O2 for the duration of the time trial distance. The high anaerobic capacity of speed skaters, one of the highest recorded by any group of athletes24, could explain the evident V̇O2 leveling off on the last stages of the incremental skating protocols. However, since anaerobic capacity was not specifically measured during the skating protocols, this speculation still needs to be addressed. The correlation found between V̇O2max expressed in L∙min-1 values attained during skating on a slide board and on a treadmill (r=0.94) was ‘almost perfect’ according to Hopkins21 criteria (Figure 3). A level of magnitude above very large (0.90) is warranted for concurrent validity 6 correlations21. There are no published studies examining the validity of a maximal incremental skating test on a slide board. Leone et al25 and Petrella et al26 found correlations of 0.69 – 0.76 when comparing V̇O2max during ice hockey aerobic field tests with similar running field tests as the criterion measure. The lower correlations found by the authors can be related to the different exercise modality adopted as a criterion test. The difference in correlation between V̇O2max expressed in L∙min-1 and mL∙kg-1∙min-1 can be related to difference in data homogeneity. As the homogeneity of the group increases, the variance decreases and the magnitude of the correlation coefficient tends to decrease. Therefore, since V̇O2max group values are less heterogeneous when expressed in mL∙kg-1∙min-1, this could partially explain the lower correlation for this index (r = 0.84). In addition to the similar V̇O2 responses and very large correlation observed for V̇O2max attained when skating on a treadmill and on a slide board, these two conditions resulted in a low between test variability of 5%. This value is similar to the one reported using supramaximal verification bouts versus graded maximal test to elicit V̇O2max (4.3%) reported by Hawkins et al.27. Thus, the slide board protocol seems to provide valid and consistent values of V̇O2max when considering account individual subject variations. Very large correlations (r > 0.80) were found between the athletes’ best time of the season in 1500 m distance and the GET (r = 0.81), RCP (r = 0.89) and V̇O2max (r = 0.91) measured on the slide board further support the validity of those indices to predict and evaluate speed skating performance on ice. This contrasts with the lack of relationship shown between aerobic and anaerobic indices obtained during a cycling test and skating performance22, supporting the notion that cycling tests are not ideal to evaluate seasonal changes in performance of highly trained skating athletes. Yet one must acknowledge that the V̇O2max was more homogeneous in van Ingen Schenau et al. 22. Despite the similarities found between treadmill and slide board skating, V̇Emax, RERmax and [La]peak were significantly smaller during the slide board test and ES were moderate to large, pointing out for meaningful differences (Table 1). These findings were unexpected and might be related to slight differences in body posture adopted on the slide board, since at smaller knee and/or hip angles the ventilatory response can be altered because of mechanical limitations during skating12, 28. De Boer et al.28 found greater RER and V̇E maximal values during inline compared to on ice maximal skating tests, with no significant differences in V̇O2max, and a more upright posture during inline skating. Differences in RER could be related to decreased V̇E, as suggested by Rundell and Pripstein12, or could be linked to a decreased [La]peak possibly caused by a more pronounced blood flow occlusion and the associated decrease in lactate efflux29. V̇O2 values at GET and RCP were similar and well correlated (Table 2) in both conditions, and represented ~ 67 and ~ 92% of V̇O2max obtained during the skating tests. Piucco et al.13 reported similar %V̇O2 values at the second ventilatory threshold, i.e. RCP, during two different skating protocols on slide board, when investigating skaters with similar profile. However, these values are higher than those reported for GET (61%) and RCP (80%) during cycling16. Boone et al.30 found RCP intensities at ~ 87% of VO2max during cycling, this value being influenced by the aerobic fitness level i.e. trained individuals showing greater percent RCP. Therefore, a high endurance capacity of the speed skaters1,7, combined with the idea that true maximal values might not have been achieved due to the blood flow restriction, could result the larger percent RCP associated to V̇O2max observed in this study. It is important to note that this is the first study investigating GET and RCP during a specific skating protocol. An effective evaluation of sport performance needs to reproduce the physiological responses during exercise, which are dependent on the characteristics of the movement pattern, such as posture, muscle recruitment and mode of contraction utilized 7 during exercise31, 32. The unique crouched skating technique (i.e. small knee- and trunk angle) leads to an increase deoxygenation of the working muscles. Due to the reduced blood flow associated with the crouched skating posture, an increased recruitment of the fast-twitch fibres may occur33. Consequently, although commonly used7, 22, cycling tests do not offer optimal conditions to evaluate physiological variables associated with performance in speed skating. Therefore, the similar values for skating intensity threshold observed during treadmill skating and slide board strengthens the validity of skating on a slide board as a good model to represent the physiological profile of skating. The main limitation of the slide board protocol is that it cannot replicate the effects of cornering. Technical aspects of cornering seem to have an impact on oxygenation, affecting processes related to the regulation of exercise intensity such as fatigue and recovery34. Also, aerodynamics, drafting effects, and optic flow perception are not important in treadmill and slide board tests, while they are important determinants of skating performance33. Keeping the skating treadmill on a level grade during the maximal treadmill skating test is another potential limitation. Skating on a level grade can be technically too difficult and might lead to the test finishing without exerting the highest levels of power due to lack of resistance and small friction. This might be especially true for recreational skaters, hockey players, cross-country skaters and amateur speed skaters. In this case, changing the treadmill inclination or using additional methods to increase skating resistance during the test is recommended. Further comparison between slide board test and field test results are necessary to strengthen the validity of the results. However, the strong correlations found between the maximal and submaximal V̇O2 data during skating on a slide board and skating performance on ice suggest that actual determinants of performance can be assessed using the slide board test. Practical Applications The V̇O2, HR and V̇E profile of skating on a slide board is similar to skating on a treadmill. Also, outcomes of the skating test on a slide board skating relate to actual skating performance. Therefore, speed skating coaches can use the slide board test as a valid and specific method to establish maximal physiological indices and exercise intensity boundaries. The accessibility of the slide board makes this tool of interest when considering repetitive testing in different locations (i.e. training camps) during the season. Conclusions In conclusion, the results of the present study support the hypothesis that skating on a slide board mimics some important physiological responses obtained during treadmill skating. The slide board test, a simple and affordable approach in terms of accessibility and cost, was capable to appropriately assess maximal and submaximal parameters that determine endurance performance, such as V̇O2max and aerobic/anaerobic intensity thresholds during skating. The slide board test is valid (correlation coefficients above 0.9) to determine V̇O2max and V̇O2 at the thresholds. However, caution should be taken when using HR and blood lactate data from slide board incremental test for training prescription. Maximal and submaximal aerobic indices during slide board skating test were correlated with skating performance on ice (i.e., best time on 1500 m skating distance). Thus, the slide board test is a specific and practical test that can be used to evaluate speed skating performance, prescribe exercise training intensities and monitor adaptations due to training program. 8 Acknowledgements The authors acknowledge the funding support of National Council for Scientific and Technological Development (CNPq) and Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil, and the athletes for participating in this study. 9 References 1. De Koning JJ, Bakker FC, de Groot G, et al. Longitudinal development of young talented speed skaters: physiological and anthropometric aspects. J Appl Physiol. 1994;77:23112317. 2. 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Validation of the FAST skating protocol to predict aerobic power in ice hockey players. Appl Physiol Nutr Metab. 2007;32(4):693700. 27. Hawkins MN, Raven PB , Snell PG, et al. Maximal oxygen uptake as a parametric measure of cardiorespiratory capacity. Med Sci Sports Exerc. 2007;39:103-107. 28. De Boer RW, Vos E, Hutter W, et al. Physiological and biomechanical comparison of roller skating and speed skating on ice. Eur J Appl Physiol. 1987;56(5):562-569. 29. Gladden LB. Muscle as a consumer of lactate. Med Sci Sports Exerc. 2000;32(4):764–771. 30. Boone J, Barstow TJ, Celie B, et al. Oxygenation, muscle activation and pulmonary VO2 to incremental ramp exercise: influence of aerobic fitness. Appl Physiol Nutr Metab. 2016;41(1):55-62. 31. Basset FA, Boulay MR. Specificity of treadmill and cycle ergometer tests in triathletes, runners and cyclists. Eur J Appl Physiol. 2000;81(3):214-221. 32. Duchateau J, Semmler JG, Enoka RM. Training adaptations in the behavior of human motor units. J Appl Physiol. 2006;101:1766–1775. 33. Konings MJ, Elferink-Gemser MT, Stoter IK, et al. Performance characteristics of longtrack speed skaters: A literature review. Sports Med. 2015;45(4):505-516. 34. Hettinga FJ, Konings MJ, Cooper CE. Differences in muscle oxygenation, perceived fatigue and recovery between long-track and short-track speed skating. Front Physiol. 2016;7:619. 11 Table 1 - The mean ± standard deviation values, Pearson’s product moment correlation coefficients (r), effect size (ES) and the relative typical error of measured (TEMCV%) between maximal variables obtained when skating on a treadmill skating and on a slide board. Treadmill Slide board r ES TEM CV% b ̇VO2max (L∙min-1) 3.04 ± 0.54 3.05 ± 0.65 0.94 0.002 5.3 V̇O2max(mL∙kg-1∙min-1) 46.7 ± 4.4 46.4 ± 6.1 0.80 b 0.05 5.5 b HRmax (bpm) 195 ± 6 196 ± 10 0.87 0.05 2.0 RERmax 1.3 ± 0.1 1.2 ± 0.1 a 0.30 1.65 5.1 a b ̇VEmax (L∙min-1) 148.5 ± 28.3 138.7 ± 22.4 0.87 0.41 6.6 [La]peak (mmol∙L-1) 12.2 ± 2.3 9.3 ± 2.3a 0.11 1.28 20.3 a - Significant difference (p < 0.05); b - Significant correlation (p < 0.05). V̇O2max = maximal oxygen uptake; HRmax = maximal heart hate; RERmax = maximal respiratory exchange ratio; V̇Emax = maximal ventilation; [La]peak = peak blood lactate concentration. 12 Table 2 - The mean ± standard deviation values, Pearson’s product moment correlation coefficients (r), effect size (ES) and the relative typical error of measured (TEMCV%) between submaximal variables (GET and RCP) obtained when skating on a treadmill and on a slide board . ES GET Treadmill Slide board r TEMCV% b ̇VO2 (L∙min-1) 2.02 ± 0.37 2.04 ± 0.36 0.91 0.06 5.5 ̇VO2 (mL∙kg30.9 ± 3.2 31.4 ± 4.1 0.75 b 0.12 6.1 1 ∙min-1) % V̇O2max 66.3 ± 4 67.7 ± 7.1 0.62 b 0.24 6.5 b HR (bpm) 153 ± 14 150 ± 12 0.90 0.29 3.1 a RER 0.92 ± 0.06 0.88± 0.04 0.50 0.78 7.7 b ̇VE (L∙min-1) 59.8 ± 11.8 57.0 ± 10.7 0.85 0.25 7.4 RCP Treadmill Slide board r ES TEMCV% b ̇VO2 (L∙min-1) 2.78 ± 0.53 2.81 ± 0.56 0.97 0.05 3.0 V̇O2 (mL∙kg42.5 ± 4.4 42.9 ± 4.8 0.92 b 0.09 3.0 1 ∙min-1) % V̇O2max 91.1 ± 3.3 92.4 ± 2.1 0.37 0.50 3.3 b HR (bpm) 178 ± 9 178 ± 6 0.85 0.04 2.0 RER 1.05 ± 0.08 0.99 ± 0.05a 0.50 0.92 4.3 -1 b V̇E (L∙min ) 96.5 ± 19.0 92.1 ± 12.7 0.90 0.27 7.2 a - Significant difference (p < 0.05); b - Significant correlation (p < 0.05). V̇O2 = oxygen uptake; % V̇O2max = percentage of V̇O2max; HR= heart hate; RER = respiratory exchange ratio; V̇E = ventilation. GET = gas exchange threshold; RCP = respiratory compensation point. 13 Figure 1. Slide board set up. 1- Photo emitter; 2- Photo receptor. Figure 2. Incremental skating protocol to exhaustion on a treadmill (A) and on a slide board (B). Increase in cadence on the slide board is represented by numbers of push-offs per minute (ppm). Figure 3. Correlation (95% CI) between V̇O2max attained on a treadmill and on a slide board. Figure 4. Average V̇O2 response during skating on a treadmill (panel A) and on a slide board (panel B). 14 15