The energy cost of running (Erun), a key determinant of distance running performance, is influenced by several factors. Although it is important to express Erun as energy cost, no study has used this approach to compare similarly trained men and women. Furthermore, the relationship between Achilles tendon (AT) stiffness and Erun has not been compared between men and women. Therefore, our purpose was to determine if sex‐specific differences in Erun and/or AT stiffness existed. Erun (kcal kg−1 km−1) was determined by indirect calorimetry at 75%, 85%, and 95% of the speed at lactate threshold (sLT) on 11 man (mean ± SEM, 35 ± 1 years, 177 ± 1 cm, 78 ± 1 kg, V˙O2max = 56 ± 1 mL kg−1 min−1)...